Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) Application: ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P. Reactivity: Mouse. DyLight 405. Alexa Fluor® 594 (NHS Ester) 584: 616: ATTO TM 590 (NHS Ester) Alexa Fluor® Dyes: ROX (NHS Ester) 588: 608: ATTO TM Rho101 (NHS Ester) Freedom™ Dyes: ATTO TM Rho101 (NHS Ester) 592: 609: ROX (NHS Ester) ATTO TM Dyes: ATTO TM 590 (NHS Ester) 593: 622: Alexa Fluor® 594 (NHS Ester) ATTO TM Dyes: TEX 615 : 596: 613: Texas Red®-X (NHS Ester) Freedom™ Dyes: Texas Red®-X (NHS Ester) … John > … MoticEurope is the European Headquarter and Distribution Centre, located in Barcelona. The image was captured by 10X objective. Alexa Fluor™ 594 is a bright, red fluorescent dye. DyLight 350. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. DyLight 488. A common filter for this channel is a 610/20 bandpass. The Texas Red light cube provides a 585/29 Ex and 624/40 Em filter set. DyLight 755. Alexa Fluor 405: 422nm : 401nm : Alexa Fluor 488™ 520nm : 498nm : Alexa Fluor 514™ 539nm : 517nm : Alexa Fluor 532™ 554nm : 531nm : Alexa Fluor 546™ 573nm : 556nm : Alexa Fluor 555™ 568nm : 553nm : Alexa Fluor 568™ 603nm : 578nm : Alexa Fluor 594™ 617nm : 590nm Available Conjugates. We are thinking about > substituting Alexa 594 for Texas Red in a four color > experiment where we would normally run FITC, PE, TxR, > and Cy5 using 488 nm and 600 nm (dye laser) excitation. BD Horizon Brilliant™ Ultraviolet 737 (BUV737) (Ex-Max 350 nm/Em-Max 732 nm) BD Horizon™ BUV737 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye … For example Alexa Fluor 488, phycoerythrin (PE), peridinin chlorophyll protein (PerCP)-Cy5.5 and PE-Texas Red can all be excited at 488 nm, but will produce green, yellow, red and infrared emissions, respectively, which can then be measured using separate detectors. Cy5. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. Hi Farid! Rhodamine red: Alexa Fluor 568: Texas Red: Alexa Fluor 594: Cy5: Alexa Fluor 647: Cy5.5, IR680: Alexa Fluor 680: Cy7: Alexa Fluor 750: With that said, Alexa dyes are often more expensive than common dyes. Alexa Fluor 594®, Texas Red® DyLight® 650 : 652/672. DyLight 550. Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L (ab125913) Specific References (6) Description: Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L. Red 594 593/618 80,000 Alexa Fluor 594 and Texas Red® Dyes Red 633 638/658 170,000 Alexa Fluor 633 Dye Red 650 652/677 250,000 Alexa Fluor 647 and Cy5 Dyes Near-IR 680 692/712 140,000 Alexa Fluor 680 and Cy5.5 Dyes Near-IR 755 754/776 220,000 Alexa Fluor 750 and Cy7 Dyes Near-IR 800 777/790 270,000 IRDye® 800 Dye The > literature from Molecular Probes says that Alexa 594 is > superior to Texas Red. If using an EVOS system, we have a Texas Red light cube that should provide excellent illumination for AF594. 250K. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor 488 Molecular Probes General conjugated label Good 495 nm 520 nm 530DF30 530DF60 Alexa Fluor 532 Molecular Probes General conjugated label Good 531 nm 554 nm 550LP/605DF50 520LP Alexa Fluor 633 Molecular Probes General conjugated label Good 631 nm 647 nm 695DF50 Not supported AttoPhos Molecular Probes General conjugated label DyLight 594. FVB/N mouse frozen thymus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature. This enables multicolor analysis in conjunction with APC or Alexa Fluor® 647, and APC-H7 or APC-Cy7 reagents. Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry. PE/Dazzle™ 594 can be excited by the blue (488 nm), green (532 nm) or yellow/green lasers (561 nm), and is detected in the PE/Texas Red® channel. Alexa Fluor™ 594 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. Alexa Fluor® 700. Recombinant. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 594 dye is water soluble and pH-insensitive from pH 4 to pH 10. I have only used Alexa 488 and 594, but in my experience they are far more photostable than FITC or Texas Red. Atto 390. Does DyLight® 550 have the same superior photostability as DyLight® 549? AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. DyLight 650. The secondary antibodies were Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (colored green) used at 2 ug/ml and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) (pseudo-colored red) used at 1/1000 dilution for 1hour at room temperature. The powder is dark purple. Alexa Fluor® 594. Atto 633 is an alternative to Alexa Fluor 633. Alexa Fluor 594 conjugates (Amax 591 nm, Emax 614 nm) emit in the red range of the visible light spectrum, are brighter than other red-fluorescing dye conjugates, and allow better color separation from green fluorescent dyes like Alexa Fluor 549, Cy3, or TRITC. Alexa Fluor® 594. They are the best choice for immunofluorescence detection in the far red region of the visible spectrum. DyLight 680. Alexa Fluor® 700 is a far-red dye that can be excited with a 633–640-nm laser. Alexa Fluor® 594 is an excellent alternative to the Texas Red dye. Molecular Probes, Inc. Alexa Fluor®, Texas Red®, and Pacific Blue™ dye antibody conjugates are sold under license from Molecular Probes, Inc., for research use only, excluding use in combination with microarrays, or as analyte specific reagents. 6. PE-Texas Red ® 566 616 Alexa Fluor®594 590 617 DyLight ®594 593 618 EviTag TM quantum dots-Maple-Red Orange 600 620 Alexa Fluor®610 612 628 Chromeo TM 494 494 628 Alexa Fluor®633 632 647 SureLight®APC 652 657 DyLight®633 638 658 Allophycocyanin (APC) 650 660 595,633,635,647 Chromeo TM 642 642 660 Quantum Red 488 660 SureLight®P3 614 662 Alexa Fluor®647 650 665 … An exceptionally bright and highly photostable deep-red fluorescent dye spectrally similar to Alexa Fluor® 594 and Texas Red®. Alexa Fluor® 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. Motic is a manufacturer of compound microscopes and digital microscopy solutions. alexa 594 vs. Texas Red John Altman altman at microbio.emory.edu Wed Dec 2 22:15:17 EST 1998. Alexa Fluor 635 is dark red, right! Compare PE/Dazzle™ 594 against other fluors with our Fluorescence Spectra Analyzer. It might solve some of the problems with Cy5PE tandems that exist on the Calibur (although I realize it would require a different set of filters). I tried the first antibody also with Texas Red as a secondary antibody and it worked fine. Rhodamine. Yes. HA.11 recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. Cy3. alexa 594 vs. Texas Red Justin Fishbaugh justin-fishbaugh at uiowa.edu Wed Dec 23 16:08:11 EST 1998. Texas Red . Previous message: ICAM-1 Next message: Magneto Optical Drive Messages sorted by: Mario, Do you know if anyone has tested a PE-Alexa-594 tandem? Alexa Fluor dyes are synthesized through sulfonation of coumarin, rhodamine, xanthene(such as fluorescein), and cyanine dyes Instead, fluorophores with absorption peaks residing at longer wavelengths, such as Alexa Fluor 568, Alexa Fluor 594, or Texas Red (578, 590, and 596-nanometer absorption maxima, respectively) should be employed with the krypton-argon laser. So I´m sure that the Alexa 350 is the problem. Alexa Fluor® 594 conjugates are brighter than red-fluorescing conjugates, and they provide more color separation from green-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. iFluor™ 594 has superior labeling performance and better stability than Texas Red®. Alexa Fluor® 546 Fura Red™ Qdot® 565 nanocrystal DyeCycle™ Orange Qdot® 585 nanocrystal SNARF® (low pH) JC- 1 or DiOC2(3) pHrodo™ dye Argon 488 Orange 616/23 604–628 RPE-Alexa Fluor® 610 PI RPE-Texas Red® JC- 1 or DiOC2(3) Qdot® 705 nanocrystal LIVE/DEAD® Fixable Red Dead Cell Stain Argon 488 Red 670LP ≥670 RPE-Alexa Fluor® 700 PI (695/40)* 7-AAD Fluorophore selection … Conjugate: Alexa Fluor® 594. Cy5.5. Atto 594 is an alternative to Alexa Fluor 594 and Texas Red. Alexa Fluor 647®, Cy5® Ex/Em: Excitation and emission wavelength in nanometers (+/- 4nm) ε: Molar extinction coefficient at the absorption maximum. iFluor™ 594 has spectral characteristics similar to those of Texas Red®, DyLight™ 594 and Alexa Fluor® 594 with excitation and emission wavelengths at ~592 nm/614 nm when conjugated to proteins. Alexa Fluor® 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. Lectins for carbohydrate binding studies. Then the section was stained with 5 µg/ml of anti-mouse CD90.1 (clone OX-7) Alexa Fluor® 594 (red) overnight at 4°C. AMCA. Texas Red fluoresces at about 615 nm, and the peak of its absorption spectrum is at 589 nm. Bioz Stars score: 98/100, based on 76 PubMed citations. Nuclei were counterstained with DAPI (green). Atto 565. The Alexa Fluor® dyes (except for Alexa Fluor® 430) and Pacific Blue™ dye are covered by pending and issued patents. The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name. Show more Hide. Atto 647N is an extraordinary highly fluorescent dye, and Atto 655 are alternatives to Cy5 and Alexa Fluor 647. ZERO BIAS - … Several of the Alexa Fluor dyes are specifically designed for excitation by either the blue diode laser (405 nanometers; see the Alexa Fluor 405 chemical structure in Figure 5(a)), the orange/yellow helium-neon laser (594 nanometers), or the red helium-neon laser (633 nanometers). DyLight 633. Alexa Fluor 594 Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Alexa Fluor 555 553 568 1250 QY 0.1 Alexa Fluor 568 579 603 792 QY 0.69 Alexa Fluor 594 591 618 820 QY 0.66 Alexa Fluor 610 610 629 1285 Alexa Fluor 633 632 648 1200 Alexa Fluor 647 652 668 1300 QY 0.33 Alexa Fluor 660 663 691 1100 Alexa Fluor 680 680 702 1150 Alexa Fluor 700 Alexa Fluor® 647. Previous message: Apoptosis assessment with PI Next message: CD69 Messages sorted by: Greetings, Back in November I inquired about using Alexa 594 in place of Texas Red and the responses were very favorable. 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