Typically the strands of the stem of the hairpin are anti-parallel. Maleimide, bormide, iodide, or sulphonyl derivatives are suitable for tagging thiol-linked oligos with a variety of groups such as fluorescent dyes, biotin, and enzymes. All Alt-R CRISPR-Cas9 crRNAs are 35–36 nt RNA oligos containing the 19 or 20 nt target-specific protospacer region, along with the 16 nt tracrRNA fusion domain. When you order GMP-manufactured oligos from IDT, you receive the highest standards of traceability. Oligos, Primers, Probes & Genes Pipettes & Pipette Tips SuperScript Reverse Transcriptase TaqMan Real-Time PCR Assays TRIzol Reagents Most Popular Categories Antibodies & Immunoassays Cell Culture & Transfection The controlled and validated process is governed by the Oligo Master Record (OMR)—our collaborative playbook for how we produce your oligos every time you order. If the oligos are going to be resuspended, storage in a TE buffer (10 mM Tris, 0.1 mM EDTA, pH 7.5–8.0; such as IDTE) is better than using water (non-DEPC treated). <>>> The Alexa Fluor 488 Oligonucleotide Amine Labeling Kit provides a simple method for labeling amine-modified oligonucleotides with our bright, photostable, green-fluorescent Alexa Fluor 488 dye (which is spectrally similar to n¢Ä_FBÅ@­­Q&Yœè(kT6F¥ªÔà ժÌ$;Feê¨T+…Ô1hTлÂÛãÉZÎóٙ]#²Åì=ÔôC^Ãôã@/«þû~DmªÇzRÏ« Zƒ¶…«{Ò ÿ. endobj Oligos Tools & Utilities Hub NEW Design, create, and assemble the tools you need for exploring genomes. However, by switching to 5'-phosphoramidites for part of the synthesis of such an oligo For optimal stability, oligos that are to be stored long term should be stored frozen, at -20°C. �Nj?��T,>�a��i�\�\�\�-W�ې�E ����#N��5~��P.V�������^����o����� >B��2T����|�cZA30lĹbiމ���O���u�/9�rƽ��HL.�\I�ɤg�q�&��e�����OWAp� �����A�%��9��d�r���qO^��E�X�4b�A"EGd_H�Xld���V�2�����T��$��{ì����oH��ș. Featured DECODED article: How to facilitate your research on the 2019 Novel Coronavirus (SARS-CoV-2) Webinar summary: Learn about IDT’s high-quality line of genomic reagents that can be used to facilitate your research of COVID-19, caused by the novel … The principles of fluorescence and the synthesis of fluorescent oligonucleotides are described below. For these reasons, IDT calculates the extinction coefficient for every oligo synthesized using a nearest neighbor method. Figure 8.2.1 A microarray hybridized to Alexa Fluor 546 dye–labeled cDNA. We strongly recommend resuspending and storing fluorescently labeled oligos in a buffered solution such as TE (10 mM Tris pH 8.0, 0.1 mM EDTA) at -20 C. This will help to increase the shelf life of your dye labeled oligos. 2 0 obj Generate reliable, high-quality gene expression analysis data with double- and single-quenched fluorescent hydrolysis probes. FAM can be attached to 5' or 3' end of oligos. Shipping in specially designed, easy-to-identify envelopes by express overnight delivery. 4 0 obj 2 Fluorescently labeled tracrRNA provides efficient genome editing while allowing cellular microscopy and FACS analysis application note genome editing Figure 1. The mini package at 50 nmol scale is especially designed for avoiding waste in a tight budget. Oligos with hairpin loops are used for structural studies into duplex formation. IDT product details for Fluorophores modifications Dye 5' Int 3' Ex. WellRED oligos use cyanine-based fluorescent dyes with high extinction coefficients that absorb in the near-infrared region, thereby reducing background noise from biological materials, resulting in highly sensitive detection. DNA Oligo Synthesis: GenScript provides modified DNA oligos with flexible synthesis scales for your individual research needs. We have found that oligos stored in water at 37 C It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. cDNA was labeled by reverse transcription from Vibrio cholerae total RNA using ChromaTide Alexa Fluor 546-14-dUTP ( C11404 ). In addition, dry ice shipments will be held from Tuesday, December 22nd until Monday, January 4th. Fluorophores are very useful and sensitive labels for biomolecules, and fluorescent labels are by far the most common labels used for synthetic oligonucleotides. Proper storage of your oligo will maximize its shelf life, allowing you to get the most use from the oligo Bulk Fluorescent oligos shipped in amber tubes to protect light sensitive oligos. <> Fluorescent dyes, Affinity Ligands, Spacers & Linkers, Duplex Stabilizers, Minor bases, labeled oligos, Molecular Beacons, siRNA, phosphonates Locked Nuc leic Acids (LNA); 2’-5’ linked Oligos IDT has tested oligos under what we consider worst-case scenario conditions— a constant 37 C—to represent oligos delayed in transit or left out of the freezer for a period of time. x��Z�n�8}7��cka�"%R��0ֱ�A����o�y�mu[��Gݎ���}Y���x���;ٙM���&Kź�:,��e�����>8? Please contact IDT Customer Care for modifications not listed A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis. Further, it is necessary to take into account the presence of oligo modifications, such as fluorescent dyes, which may have significant absorbance at 260 nm. Oligos are delivered lyophilized in individual vials or in 96 or 384 well plates. IDT develops, manufactures, and markets nucleic acid products that support the life sciences industry in the areas of academic and commercial research, agriculture, medical diagnostics, and pharmaceutical development. ?������!�|������og��O�٧bUm�}Uo/.�w�W���㣳"�˃T�H$��,K�� ��& V��(X�]f�~8>����O�" Normal business hours and shipping will resume on Monday the 28th. endobj 3 0 obj Pair with ZEN™ and Iowa Black™ quenchers to create probes with lower background and higher signal. stream metabion provides his customers with high quality DNA- and RNA-Oligos e.g. The streptavidin can be labeled with fluorescent dyes and enzymes or mediate attachment to a solid surface. for real-time PCR and with a wide variety of Life Science Products like Peptides, … 1 0 obj ATTO 700 has characteristic features of the label are strong absorption-20 C To -70 C Oligonucleotides are stable in solution at 4 C for up to 2 weeks. %���� BioVision’s Fluorescent Dye Labeled siRNA Oligos can be labeled in the four different ends of double stranded by multiple markers. At no extra The major applications of fluorescent and fluorogenic oligonucleo… Bio-Synthesis offers ATTO 700 fluorescent dye oligonucleotide labeling services. A variety of molecular biology assays and purification methods employ Addition of ATTO 550 fluorescent … FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. License-free use in commercial or diagnostic applications. Proprietary IDT fluorophores for commonly used dye wavelengths. <>/ExtGState<>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/Annots[ 15 0 R] /MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> We recommend 20 nt protospacers for most applications IDT will also be closed on December 31st and January 1st for the New Year. IDT uses a proprietary desalting technique that removes some truncation products and small organic contaminants from the synthesized oligonucleotide preparation. Alternative Category Alexa Fluor® 488 (NHS Ester) 492 517 ATTO TM 488 (NHS Ester) Alexa Fluor® Dyes 6-FAM (Fluorescein) 495 520 N/A Freedom Dyes Removal of n-1mers produced during synthesis requires additional PAGE or HPLC purification. Many different fluorophores can be readily attached to oligonucleotides and they form the basis of the detection systems used in DNA sequencing, forensic science and genetic analysis. Our diverse fluorescent labels provide the ideal tools for multicolor techniques such as spectral karyotyping, multilocus FISH analysis, chromosome painting and comparative genome hybridization. Our product offering includes common dye modifiers such as 6-FAM , HEX , and TET , as well as exotic modifications. Each oligo is accompanied by a spec sheet listing your sequence name, sequence, MW, G/C content, Tm, OD, mg and nanomole yield. Other Black Hole As the maleimide moiety itself is not stable over the long-term, Gene Link provides lyophilized maleimido-oligos with all base protecting groups removed and as a maleimide-2,5-dimethylfuran cycloadduct.The cycloadduct serves to protect the maleimide from degradation. Biotin-modified oligos bind tightly to streptavidin. A single isomer derivative of fluorescein. Oligonucleotide Handling & Stability is a techniques in order to ensure trouble-free experiments. They are widely recognized as the industry leader in the manufacture of custom oligonucleotides for molecular biology applications. <> The labeled siRNA oligos can be monitored by Flow Cytometry, fluorescence microscope, laser co-focus microscope and so on to determine transfection efficiency and optimize Transfection conditions. Award winning Technical Services for complete workflow support. When possible, this is done using a modified solid support (CPG) for 3'-modifications or a specialized … %PDF-1.5 Inventoried oligos GMP & OEM Services Custom qPCR probes qPCR probe design and production quality are paramount in creating specific qPCR assays with robust signal-to-noise performance. Modifications available at IDT include for oligonucleotide attachment include: I-LinkerTM Amine-modified oligos covalently linked to an activated carboxylate group or succinimidyl ester Thiol-modified oligos … Choose from 5′-labeled fluorescent primers and primer pairs for use in fragment analysis on the capillary electrophoresis (CE) platform. Includes fluorescein, Cy ® (Cytiva), rhodamine, and ATTO™ dye categories. endobj 3050 Spruce Street, St. Louis, MO 63103 USA Tel: (800) 521-8956 (314) 771-5765 Fax: (800) 325-5052 (314) 771-5757 1 Custom Oligonucleotide Modifications Guide … Em. We can attach fluorescent dyes to oligos at the 5'-end, 3'-end and internal positions. IDT will be closed Thursday, December 24th and Friday the 25th, for the Christmas holiday and on December 31st and January 1st for the New Year. Thiol-modified siRNA and RNA oligonucleotides with the PrimeTime qPCR Probes are 5′ nuclease probes, available with an assortment of reporter-dye combinations that are compatible with common qPCR instruments and that include several license-free combinations. Maleimide-labeled oligos are typically used to form conjugates with thiol-labeled moieties, or Diels-Alder cycloaddition products. IDT will be closed on Friday the 25th, for the Christmas holiday. Allowing cellular microscopy and FACS analysis application note genome editing while allowing cellular microscopy and FACS analysis application note editing... Details for Fluorophores modifications dye 5 ' Int 3 ' end of oligos most fluorescence detection equipment synthesis additional. 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